Please use this identifier to cite or link to this item:
http://digitalrepository.fccollege.edu.pk/handle/123456789/1038
Title: | Azospirillum zeae sp. nov., a diazotrophic bacterium isolated from rhizosphere soil of Zea mays |
Authors: | Mehnaz, Samina Weselowski, Brian Lazarovits, George |
Keywords: | Azospirillum / chemistry Bacterial Proteins / genetics DNA, Bacterial / genetics Microscopy, Electron, Transmission Nitrogen Fixation / physiology Zea mays / microbiology* Oxidoreductases / genetics Sequence Analysis, DNA |
Issue Date: | 1-Dec-2007 |
Publisher: | PubMed.gov - National Library of Medicine, National Center of Biotechnology Information |
Citation: | Mehnaz S, Weselowski B, Lazarovits G. Azospirillum zeae sp. nov., a diazotrophic bacterium isolated from rhizosphere soil of Zea mays. Int J Syst Evol Microbiol. 2007 Dec;57(Pt 12):2805-2809. doi: 10.1099/ijs.0.65128-0. PMID: 18048728. |
Series/Report no.: | Int J Syst Evol Microbiol . 2007 Dec;57(Pt 12):2805-2809;PMID: 18048728 |
Abstract: | Two free-living nitrogen-fixing bacterial strains, N6 and N7(T), were isolated from corn rhizosphere. A polyphasic taxonomic approach, including morphological characterization, Biolog analysis, DNA-DNA hybridization, and 16S rRNA, cpn60 and nifH gene sequence analysis, was taken to analyse the two strains. 16S rRNA gene sequence analysis indicated that strains N6 and N7(T) both belonged to the genus Azospirillum and were closely related to Azospirillum oryzae (98.7 and 98.8 % similarity, respectively) and Azospirillum lipoferum (97.5 and 97.6 % similarity, respectively). DNA-DNA hybridization of strains N6 and N7(T) showed reassociation values of 48 and 37 %, respectively, with A. oryzae and 43 % with A. lipoferum. Sequences of the nifH and cpn60 genes of both strains showed 99 and approximately 95 % similarity, respectively, with those of A. oryzae. Chemotaxonomic characteristics (Q-10 as quinone system, 18 : 1omega7c as major fatty acid) and G+C content of the DNA (67.6 mol%) were also similar to those of members of the genus Azospirillum. Gene sequences and Biolog and fatty acid analysis showed that strains N6 and N7(T) differed from the closely related species A. lipoferum and A. oryzae. On the basis of these results, it is proposed that these nitrogen-fixing strains represent a novel species. The name Azospirillum zeae sp. nov. is suggested, with N7(T) (=NCCB 100147(T)=LMG 23989(T)) as the type strain. |
URI: | http://localhost:8080/xmlui/handle/123456789/1038 |
Appears in Collections: | School of Life Sciences |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
N7.IJSEM.pdf | 127.63 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.