Please use this identifier to cite or link to this item: http://digitalrepository.fccollege.edu.pk/handle/123456789/1058
Title: Secondary Metabolites Production and Plant Growth Promotion by Pseudomonas chlororaphis and P. aurantiaca Strains Isolated from Cactus, Cotton, and Para Grass
Authors: Shahid, Izzah
Rizwan, Muhammad
Baig, Deeba Noreen
Saleem, Rahman Shahzaib
Malik, Dr. Kauser .A.
Mehnaz, Samina
Keywords: Phenazines
Pseudomonas aurantiaca
Pseudomonas chlororaphis
lahorenoic acid
white line-inducing principle (WLIP
zinc solubilization.
Issue Date: 14-Dec-2016
Publisher: jmb
Citation: Shahid I, Rizwan M, Baig DN, Saleem RS, Malik KA, Mehnaz S. Secondary Metabolites Production and Plant Growth Promotion by Pseudomonas chlororaphis and P. aurantiaca Strains Isolated from Cactus, Cotton, and Para Grass. J Microbiol Biotechnol. 2017 Mar 28;27(3):480-491. doi: 10.4014/jmb.1601.01021. PMID: 27974729.
Series/Report no.: J Microbiol Biotechnol . 2017 Mar 28;27(3):480-491;
Abstract: Fluorescent pseudomonads have been isolated from halophytes, mesophytes, and xerophytes of Pakistan. Among these, eight isolates, GS-1, GS-3, GS-4, GS-6, GS-7, FS-2 (cactus), ARS-38 (cotton), and RP-4 (para grass), showed antifungal activity and were selected for detailed study. Based on biochemical tests and 16S rRNA gene sequences, these were identified as strains of P. chlororaphis subsp. chlororaphis and aurantiaca. Secondary metabolites of these strains were analyzed by LC-MS. Phenazine-1-carboxylic acid (PCA), 2-hydroxy-phenazine, Cyclic Lipopeptide (white line-inducing principle (WLIP)), and lahorenoic acid A were detected in variable amounts in these strains. P. aurantiaca PB-St2 was used as a reference as it is known for the production of these compounds. The phzO and PCA genes were amplified to assure that production of these compounds is not an artifact. Indole acetic acid production was confirmed and quantified by HPLC. HCN and siderophore production by all strains was observed by plate assays. These strains did not solubilize phosphate, but five strains were positive for zinc solubilization. Wheat seedlings were inoculated with these strains to observe their effect on plant growth. P. aurantiaca strains PB-St2 and GS-6 and P. chlororaphis RP-4 significantly increased both root and shoot dry weights, as compared with uninoculated plants. However, P. aurantiaca strains FS-2 and ARS-38 significantly increased root and shoot dry weights, respectively. All strains except PB-St2 and ARS-38 significantly increased the root length. This is the first report of the isolation of P. aurantiaca from cotton and cactus, P. chlororaphis from para grass, WLIP and lahorenoic acid A production by P. chlororaphis, and zinc solubilization by P. chlororaphis and P. aurantiaca.
Description: Affiliations 1Department of Biological Sciences, Forman Christian College (A Chartered University), Lahore 54600, Pakistan. 2Applied Chemistry Research Center, Pakistan Council of Scientific and Industrial Research, Lahore 54600, Pakistan. 3Department of Chemistry, School of Science and Engineering, Lahore University of Management Sciences, Lahore 54792, Pakistan.
URI: http://localhost:8080/xmlui/handle/123456789/1058
Appears in Collections:School of Life Sciences

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