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Title: | Ferric-Pyoverdine Recognition by Fpv Outer Membrane Proteins of Pseudomonas protegens Pf-5 |
Authors: | Hartney, Sierra L. Mazurier, Sylvie Girard, Maëva K. Mehnaz, Samina Davis II, Edward W. Gross, Harald Lemanceau, , Philippe Loper, Joyce E. |
Keywords: | Proteins Pseudomonas Ferric-Pyoverdine protegens |
Issue Date: | 7-Dec-2012 |
Publisher: | Journals.ASM.org |
Citation: | J. Bacteriol. 2013, 195(4):765. DOI: 10.1128/JB.01639-12. |
Series/Report no.: | J. Bacteriol. 2013, 195(4):765.; |
Abstract: | The soil bacterium Pseudomonas protegens Pf-5 (previously called P. fluorescens Pf-5) produces two siderophores, enantio-pyo chelin and a compound in the large and diverse pyoverdine family. Using high-resolution mass spectroscopy, we determined the structure of the pyoverdine produced by Pf-5. In addition to producing its own siderophores, Pf-5 also utilizes ferric complexes of some pyoverdines produced by other strains of Pseudomonas spp. as sources of iron. Previously, phylogenetic analysis of the 45 TonB-dependent outer membrane proteins in Pf-5 indicated that six are in a well-supported clade with ferric-pyoverdine re ceptors (Fpvs) from other Pseudomonas spp. We used a combination of phylogenetics, bioinformatics, mutagenesis, pyoverdine structural determinations, and cross-feeding bioassays to assign specific ferric-pyoverdine substrates to each of the six Fpvs of Pf-5. We identified at least one ferric-pyoverdine that was taken up by each of the six Fpvs of Pf-5. Functional redundancy of the Pf-5 Fpvs was also apparent, with some ferric-pyoverdines taken up by all mutants with a single Fpv deletion but not by a mutant having deletions in two of the Fpv-encoding genes. Finally, we demonstrated that phylogenetically related Fpvs take up ferric complexes of structurally related pyoverdines, thereby establishing structure-function relationships that can be employed in the future to predict the pyoverdine substrates of Fpvs in other Pseudomonas spp. |
URI: | http://localhost:8080/xmlui/handle/123456789/1089 |
Appears in Collections: | School of Life Sciences |
Files in This Item:
File | Description | Size | Format | |
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Pyoverdin. J Bacteriology.2013.pdf | 1.65 MB | Adobe PDF | View/Open |
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