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http://digitalrepository.fccollege.edu.pk/handle/123456789/1313
Title: | Studies on N2-fixing bacteria associated with the salt-tolerant grass,Leptochloa fusca (L.) Kunth |
Authors: | Zafar, Y. Malik, Dr. Kauser .A. MIRCEN, E. G. Niemann |
Keywords: | nitrogen fixing |
Issue Date: | Mar-1987 |
Publisher: | springer link |
Citation: | Zafar, Y., Malik, K.A. & Niemann, E.G. Studies on N2-fixing bacteria associated with the salt-tolerant grass,Leptochloa fusca (L.) Kunth. Mircen Journal 3, 45–55 (1987). https://doi.org/10.1007/BF01090494 |
Series/Report no.: | Mircen Journal 3, 45–55 (1987).; |
Abstract: | N2-fixing bacteria were isolated from the rhizosphere of naturally grown salt tolerant grass (Leptochloa fusca). A broad spectrum of diazotrophs was found to be associated with the roots ofL. fusca. the systematic position of the three isolates, NIAB-1, C-2 and Iso-2 was determined by morphological, biochemical and mol % (G+C) DNA contents. Two isolates were identified asKlebsiella pneumoniae (NIAB-1) andBeijerinckia sp. (Iso-2).15N enrichment studies confirmed the nitrogen fixing ability of the isolates. The effects of different levels of combined nitrogen (NO −3 & NH +4), pH (5.5–9.0) and salt (NaCl) on nitrogenase activity of the isolates were determined at various time intervals. All isolates exhibited nitrogenase activity even in the presence of 5 mmol/l NO −3 or NH +4 in a semi-solid medium after 24 h of growth. Maximum nitrogenase activity was observed at alkaline pH and all isolates were able to tolerate up to 3% NaCl in the medium |
Description: | https://link.springer.com/article/10.1007/BF01090494#citeas |
URI: | http://localhost:8080/xmlui/handle/123456789/1313 |
Appears in Collections: | School of Life Sciences |
Files in This Item:
File | Description | Size | Format | |
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Studies on N2-fixing bacteria associated with the salt-tolerant grass,Leptochloa fusca (L.) Kunth.pdf | 4.41 MB | Adobe PDF | View/Open |
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