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http://digitalrepository.fccollege.edu.pk/handle/123456789/1344
Title: | Cloning of structural genes for ß-glucosidase fromCellulomonas biazotea intoE. coli andSaccharomyces cerevisiae using shuttle vector pBLU-D |
Authors: | Rajoka, Muhammad I. Parvez, S. Malik, Dr. Kauser .A. |
Keywords: | cloning structural genes biazotea |
Issue Date: | Nov-1992 |
Publisher: | springer link |
Citation: | Rajoka, M.I., Parvez, S. & Malik, K.A. Cloning of structural genes for ß-glucosidase fromCellulomonas biazotea intoE. coli andSaccharomyces cerevisiae using shuttle vector pBLU-D. Biotechnol Lett 14, 1001–1006 (1992). https://doi.org/10.1007/BF01021048 |
Series/Report no.: | Biotechnol Lett 14, 1001–1006 (1992).; |
Abstract: | A Smal genomic library fromCellulomonas biazotea NIAB 442 was constructed inEscherichia coli HB101 using shuttle vector pBLU-D. Three clones with ability to hydrolyse esculin were isolated. These clones were comparedin vivo andin vitro tests to select for hyper-secretion of ß-glucosidase. The recombinant plasmids were transformed to competent cells of a Ciro yeast.In vivo studies indicated that the genes were fully expressed in yeast as well. |
Description: | https://doi.org/10.1007/BF01021048 |
URI: | http://localhost:8080/xmlui/handle/123456789/1344 |
Appears in Collections: | School of Life Sciences |
Files in This Item:
File | Description | Size | Format | |
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clonning of structural genes for B glucosedase.pdf | 1.68 MB | Adobe PDF | View/Open |
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