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http://digitalrepository.fccollege.edu.pk/handle/123456789/1388
Title: | γ-ray induced mutagenesis ofCellulomonas biazotea for improved production of cellulases |
Authors: | Rajoka, MI Hussain, SRS Malik, Dr. Kauser .A. Bashir, Dr. Aftab |
Keywords: | induced muatagenesis |
Issue Date: | 1998 |
Publisher: | Springer Netherlands |
Citation: | γ-ray induced mutagenesis ofCellulomonas biazotea for improved production of cellulases ,,, Journal Folia Microbiologica Volume 43 Issue 1 Pages 15-22 |
Series/Report no.: | Journal Folia Microbiologica Volume 43 Issue 1 Pages 15-22; |
Abstract: | A rifampin-resistant mutant ofCellulomonas biazotea secreted elevated levels of cellulasesin vivo. The cellulase production in the mutant was not inhibited in the presence of 5% glucose, cellobiose or glycerol in the solid medium. The mutant exhibited approximately two- to three-fold enhanced product yields and productivity of cellular β-glucosidase over the wild parent in shake-flask culture studies when grown on either cellulosic or lignocellulosic substrates. Extracellular production of filter paper cellulase (FPase) and endo-glucanase (CMCase) were also significantly (p≤0.05) altered. During growth of the mutant on α-cellulose, the maximum volumetric productivities for CMCase, FPase and β-glucosidase were 52, 23.3, and 15.2 IUL−1 h−1,i.e 118, 121, and 229% their respective values for the parental strain. Some enzyme properties of the mutant cellulases were altered. Mutant-derived cellulases … |
Description: | https://scholar.google.com/citations?user=tlcSGfwAAAAJ&hl=en |
URI: | http://localhost:8080/xmlui/handle/123456789/1388 |
Appears in Collections: | School of Life Sciences |
Files in This Item:
File | Description | Size | Format | |
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L-Ray induced mutagenesis of cellulomonas biazotea.pdf | 3.6 MB | Adobe PDF | View/Open |
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