Profilin (pfn) isoforms transcriptional and bioinformatic exploration and Mus musculus brain tissues development

Abstract

Profilin (pfn) is an essential actin binding, post synaptic protein. Among its multiple isoforms, two (pfn1 and pfn2) have been reported in various organs including brain. pfn3 has been documented in kidneys and testes only, whereas pfn4 exclusively in testes. In this study, we report the presence of pfn isoforms, pfn3 and pfn4 in Mus musculus brain in addition to pfn1 and pfn2. Nine developmental stages were grouped into three classes, group 1: E10.5, E14.5, and E18.5 (prenatal), group 2: P0.5, P3.5, P5.5, P10.5 (postnatal) and group 3: P20.5,

P60.5 (adult). Remarkably, all four isoforms exhibited variable yet significant expression in all discerning de- velopmental stages. pfn3 presented high levels of transcription while pfn4 was transcribed minimally throughout

all stages except P3.5. Computational prediction of these full length transcripts of pfn encoding genes reveals dynamic secondary structure and pseudoknots configurations. Conclusively, transcriptomic levels are greatly connected with compact base pairing association of their secondary structures. Codon usage bias of mRNA transcripts and string analysis were additionally carried out to analyze different attributes of pfn isoforms.