1. Forman Digital Repository
  2. Forman Christian College (A Chartered University)
  3. Office of Research, Innovation and Commercialization (ORIC)
  4. Chemistry Department
Please use this identifier to cite or link to this item: http://digitalrepository.fccollege.edu.pk/handle/123456789/2259
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSaeed Iqbal, Muhammad-
dc.contributor.authorSharif, S.-
dc.contributor.authorKhan, I. U.-
dc.contributor.authorAshfaq, M.-
dc.contributor.authorAhmad, S.-
dc.date.accessioned2024-05-25T19:38:51Z-
dc.date.available2024-05-25T19:38:51Z-
dc.date.issued2010-01-19-
dc.identifier.citationSharif, Shahzad & Khan, Ihsan & Ashfaq, Muhammad & Iqbal, Mohammad & Ahmad, Saeed. (2010). Development and validation of a high performance liquid chromatographic method for the simultaneous determination of potassium clavulanate and cefadroxil in synthetically prepared tablets. Journal of Analytical Chemistry. 65. 1029-1034. 10.1134/S1061934810100072.en_US
dc.identifier.urihttp://202.142.177.21/handle/123456789/2259-
dc.descriptionCefadroxil (Fig. 1) is a semi synthetic cepha losporin antibiotic for oral administration with a chemical name (6R,7R) 7 [[(2R) 2 Amino 2 (4 hy droxyphenyl)acetyl]amino] 3 methyl 8 oxo 5 thia 1 azabicyclo[4.2.0]oct 2 ene 2 carboxylic acid mono hydrate. It is used to treat different types of bacterial infec tions such as bronchitis, tonsillitis, ear and skin infections, gonorrhea, and urinary tract infections [1]. Potassium clavulanate (Fig. 2) is a white to off white powder pro duced by fermentation of streptomyces clavuligerus and chemically designated as (2R,3Z,5R) 3 (2 hydroxyeth ylidene) 7 oxa 1 azabicyclo [3.2.0] heptane 2 carboxy late. It is potent inhibitor of β lactamase enzyme and is mostly formulated in combination with antibiotics and is usually supplied mixed with Avicel (Microcrystalline cel lulose), Syloid 244 (Colloidal silicon dioxide) [2]. The combination of potassium calvulanate with either ce fixime or cefadroxil has recently been approved by Central Drugs Standard Control Organization India [3]. Because of additive effects, some pharmaceutical companies are pursuing to launch combination of po tassium calvulanate with either cefixime or cefadroxil. In the first phase of our study, we reported an HPLC method for a synthetic binary mixture of cefixime and potassium clavulanate [4]. In the second phase we analysed binary synthetically prepared tablets of ce fadroxil and potassium calvulanate by HPLC and its method development and validation studies are re porting here.en_US
dc.description.abstractA simple, sensitive and rapid high performance liquid chromatographic method was developed and validated for the simultaneous determination of potassium clavulanate and cefadroxil in synthetically prepared tablets. Chromatographic separation and detection was carried out on a C18 column using 0.05 M potassium dihydrogen phosphate buffer (pH 5.0) and acetonitrile in the ratio of 94 : 06 (v/v) as mobile phase at wavelength of 225 nm. The method was linear in the concentration range of 3.75–22.5 μg/mL for potassium clavulanate and 15–90 μg/mL for cefadroxil. The flow rate was 1.0 mL/min and the total analysis time was less than 10 min. The mean recoveries was found to be greater than 99% with RSD less than 1.0%. The proposed method was validated by performing linearity, recovery, specificity, robustness, LOD/LOQ and within day and between day precision. The chromatographic results obtained from the synthetically prepared tablets show that the method is highly precise and accurate for the simultaneous quantitation of clavulanate potassium and cefadroxil.en_US
dc.language.isoen_USen_US
dc.publisherresearchgate.neten_US
dc.subjecthigh performance liquid chromatografia, potassium clavulanate, cefadroxil.en_US
dc.titleDEVELOPMENT AND VALIDATION OF A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF POTASSIUM CLAVULANATE AND CEFADROXIL IN SYNTHETICALLY PREPARED TABLETSen_US
dc.typeArticleen_US
Appears in Collections:Chemistry Department

Files in This Item:
File Description SizeFormat 
ANK2010.pdf165.73 kBAdobe PDFView/Open
Show simple item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.