DEVELOPMENT OF EFFICIENT MICROPROPAGATION SYSTEM FOR E. camaldulensis WITH RESPECT TO AGE OF EXPLANTS

Abstract

Efficient regeneration protocol is important for mass propagation, genetic transformation and germplasm alteration of desired plants. Thus, development of an efficient protocol is of the considerable importance for further genetic manipulation. In vitro plant regeneration was studied from two age groups of seedlings (2-11 weeks and 12-21 weeks old) by using their cotyledons, hypocotyls & leaf segments as explants. Shoot organogenesis was evaluated by using different explants on MS medium supplemented with varying concentrations of phtyohormones (1mg/l BAP ; 0.2 mg/l BAP + 1mg/l Zn ; 0.5mg/l BAP + 0.1mg/l NAA; 1.5mg/l BAP + 0.5mg/l NAA). Similarly, rooting of regenerated shoots was optimized on different concentrations of IBA (0.1mg/l;0.5mg/l;1mg/l). Hypocotyls of two to eleven weeks old seedlings as explants showed the best results of direct organogenesis on BAP (0.5mg/l) and NAA (0.1mg/l) in 21 days. However, BAP (0.2 mg/l) and Zn (1mg/l) showed no growth. Indole-3-butyric acid (1 mg/l) induced rooting within a minimum period of 12 days. Overall, rate of shoot and root formation was found to be 50%, respectively. Successfully, the platelets were acclimatized in net house and further transferred to open field conditions.